Kot, Witold Piotr3; Hammer, Karin4; Neve, Horst6; Vogensen, Finn Kvist3
1 Department of Systems Biology, Technical University of Denmark2 Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark3 University of Copenhagen4 Metabolic Signaling and Regulation, Department of Biotechnology and Biomedicine, Technical University of Denmark5 Max Rubner Institut6 Max Rubner Institut
Two phages, P793 and ΦLN04, sharing 80.1% nucleotide sequence identity but having different strains of Leuconostoc pseudomesenteroides as hosts, were selected for identification of the host determinant gene. Construction of chimeric phages leading to the expected switch in host range identified the host determinant genes as ORF21P793/ORF23ΦLN04. The genes are located in the tail structural module and have low sequence similarity at the distal end.
Applied and Environmental Microbiology, 2013, Vol 79, Issue 10, p. 3311-3314