Matos, E.3; Silva, Tomé Santos4; Wulff, Tune5; Valente, L. M. P.6; Sousa, V.6; Sampaio, E.6; Goncalves, A.7; Silva, J. M. G.6; Guedes de Pinho, P.6; Dinis, M. T.3; Rodrigues, P. M.3; Dias, J.3
1 National Food Institute, Technical University of Denmark2 Division of Industrial Food Research, National Food Institute, Technical University of Denmark3 University of Algarve4 National Institute of Aquatic Resources, Technical University of Denmark5 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6 University of Porto7 Unity of Innovation of Fish and Aquaculture Products
Maslinic acid, a natural triterpene, was evaluated as a dietary supplement to modulate glycogen post-mortem mobilization in gilthead seabream muscle. For this purpose, a multidisciplinary trial was undertaken, where flesh quality criteria, as well as biochemical and histological parameters, enzymatic activities and protein expression in the muscle were assessed. Supplementing gilthead seabream diets with maslinic acid mainly resulted in hypertrophy of muscle fibres and inhibition of cathepsin B activity, with no observed differences in terms of glycogen and ATP content of the muscle, as well as glycogen phosphorylase activity. Proteomic analysis showed a low impact of maslinic acid supplementation on muscle metabolism, with most changes reflecting increased stress coping capacity and muscle hypertrophy in maslinic acid-fed fish. As a finishing strategy to improve the muscle's energetic status, the usefulness of maslinic acid seems limited. However, it shows potential for promoting muscle growth in this species.