Rasmusson, Allan7; Hahn, Ute8; Larsen, Jytte Overgaard6; Gundersen, Hans Jørgen Gottlieb5; Jensen, Eva B. Vedel8; Nyengaard, Jens Randel7
1 Department of Mathematics - Centre for Stochastic Geometry and Advanced Bioimaging, Department of Mathematics, Science and Technology, Aarhus University2 Department of Computer Science, Science and Technology, Aarhus University3 Department of Clinical Medicine - Stereological Research Laboratory, Department of Clinical Medicine, Health, Aarhus University4 Department of Mathematics, Science and Technology, Aarhus University5 Stereological Research Laboratory, Faculty of Health Sciences, Aarhus University, Aarhus University6 Institut for Neurovidenskab og Farmakologi, Københavns Universitet7 Department of Clinical Medicine - Stereological Research Laboratory, Department of Clinical Medicine, Health, Aarhus University8 Department of Mathematics, Science and Technology, Aarhus University
This paper presents a new local volume estimator, the spatial rotator, which is based on measurements on a virtual 3D probe, using computer assisted microscopy. The basic design of the probe builds upon the rotator principle which requires only a few manual intersection markings, thus making the spatial rotator fast to use. Since a 3D probe is involved, it is expected that the spatial rotator will be more efficient than the the nucleator and the planar rotator, which are based on measurements in a single plane. An extensive simulation study shows that the spatial rotator may be more efficient than the traditional local volume estimators. Furthermore, the spatial rotator can be seen as a further development of the Cavalieri estimator, which does not require randomization of sectioning or viewing direction. The tissue may thus be sectioned in any arbitrary direction, making it easy to identify the specific tissue region under study. In order to use the spatial rotator in practice, however, it is necessary to be able to identify intersection points between cell boundaries and test rays in a series of parallel focal planes, also at the peripheral parts of the cell boundaries. In cases where over- and underprojection phenomena are not negligible, they should therefore be corrected for if the spatial rotator is to be applied. If such a correction is not possible, it is needed to avoid these phenomena by using microscopy with increased resolution in the focal plane.
Journal of Microscopy, 2013, Vol 250, Issue 2, p. 88-100
Cavalieri; local stereology; particle volume; rotator; virtual probe