1 BioLabChip, Department of Micro- and Nanotechnology, Technical University of Denmark2 Department of Micro- and Nanotechnology, Technical University of Denmark3 National Veterinary Institute, Technical University of Denmark4 Section for Public sector service and commercial diagnostics, National Veterinary Institute, Technical University of Denmark5 National Food Institute, Technical University of Denmark6 Division of Food Microbiology, National Food Institute, Technical University of Denmark7 EV Group GmbH DI8 Microsystems9 Biotools B&M Labs
Reagent pre-storage in a microfluidic chip can enhance operator convenience, simplify the system design, reduce the cost of storage and shipment, and avoid the risk of cross-contamination. Although dry reagents have long been used in lateral flow immunoassays, they have rarely been used for nucleic acid-based point-of-care (POC) assays due to the lack of reliable techniques to dehydrate and store fragile molecules involved in the reaction. In this study, we describe a simple and efficient method for prolonged on-chip storage of PCR reagents. The method is based on gelification of all reagents required for PCR as a ready-to-use product. The approach was successfully implemented in a lab-on-a-foil system, and the gelification process was automated for mass production. Integration of reagents on-chip by gelification greatly facilitated the development of easy-to-use lab-on-a-chip (LOC) devices for fast and cost-effective POC analysis.
Lab on a Chip, 2013, Vol 13, Issue 8, p. 1509-1514