1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark3 Nano Bio Integrated Systems, Department of Micro- and Nanotechnology, Technical University of Denmark4 unknown5 Politecnico di Milano6 Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Center, Technical University of Denmark7 Copenhagen Center for Health Technology, Center, Technical University of Denmark8 Politecnico di Milano
A novel potentiostat containing 54 current amplifiers matched to an array of custom-fabricated 5μm microelectrodes for electrochemical imaging of released neurotransmitters is presented. The board is integrated with a programmable microfluidic cell culture system and the whole assembly is thin and compact enough to be placed under the objective of a standard microscope for simultaneous optical and electrochemical monitoring. Each channel, scanned every 54μs, features 3pA current resolution over a 5kHz bandwidth, suitable for detecting single exocytotic events. The design and electrical characterization of the system are reported together with its functionality, certified by a 54-pixel electrochemical imaging of the diffusion of a 10μl droplet of a target analyte inside the cell culture chamber.
Proceedings of Ieee Biomedical Circuits and Systems Conference, 2012