Bojer, Martin Saxtorph2; Struve, Carsten3; Ingmer, Hanne4; Krogfelt, Karen Angeliki3
1 Food Safety and Zoonoses, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, Københavns Universitet2 Institut for Natur, Systemer og Modeller3 Statens Serum Institut4 Food Safety and Zoonoses, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, Københavns Universitet
The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.
Research in Microbiology, 2013, Vol 164, Issue 3, p. 205-210