Kongsted, Hanne3; Jonach, Beata Renata1; Kokotovic, Branko1; Nielsen, J. P.4
1 National Veterinary Institute, Technical University of Denmark2 Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark3 Danish Agriculture and Food Council4 University of Copenhagen
Objective: To evaluate associations between gross and histopathological lesions and the presence of Clostridium perfringens type A (CpA) and beta-2 toxin in piglets from 4 herds with outbreaks of diarrhoea. Methods: Pathological examinations on 51 diarrhoeic piglets aged 3-7 days were carried out. CpA was cultured and typed by PCR. A Clostridium perfringens spp targeted probe was used for flourescent in situ hybridization (FISH) and detection of beta-2 toxin in intestinal contents was performed by enzyme-linked immunosorbent assay (ELISA). Results: Piglets without intestinal lesions had a significantly (P < 0.05) higher prevalence of CpA and beta-2 toxin (Table 1).
Main Research Area:
International Meeting on Emerging Diseases and Surveillance (IMED 2013)