Zanivan, Sara3; Meves, Alexander4; Behrendt, Kristina5; Schoof, Erwin1; Neilson, Lisa J.5; Cox, Jürgen5; Tang, Hao R.3; Kalna, Gabriela3; van Ree, Janine H.4; van Deursen, Jan M.4; Trempus, Carol S.6; Machesky, Laura M.3; Linding, Rune1; Wickström, Sara A.5; Fässler, Reinhard5; Mann, Matthias7
1 Department of Systems Biology, Technical University of Denmark2 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark3 Beatson Institute for Cancer Research4 Mayo Clinic College of Medicine5 Max Planck Institute6 National Institute of Environmental Health Sciences Research7 University of Copenhagen
SILAC technology in combination with high-resolution mass spectrometry (MS) can be successfully used to measure phosphoproteomes in vivo. Here, Zanivan, Mann, and colleagues have applied SILAC-based MS to investigate phosphoproteomic changes during skin carcinogenesis, using the DMBA/TPA two-stage mouse model. Using this approach, the authors have revealed the phosphoproteomic dynamics that accompany skin cancer progression and predict specific kinase activities associated with tumor malignancy.