Tsushima, Hanako2; Malabarba, Maria Grazia3; Confalonieri, Stefano3; Senic-Matuglia, Francesca3; Verhoef, Lisette G G C3; Bartocci, Cristina3; D'Ario, Giovanni3; Cocito, Andrea3; Di Fiore, Pier Paolo3; Salcini, Anna Elisabetta5
1 Salcini Group, BRIC Research Groups, BRIC, Københavns Universitet2 IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan, Italy.3 unknown4 Salcini Group, BRIC, Faculty of Health and Medical Sciences, Københavns Universitet5 Salcini Group, BRIC, Faculty of Health and Medical Sciences, Københavns Universitet
Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of "territories" of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.
Plos One, 2013, Vol 8, Issue 2
Adaptor Proteins, Vesicular Transport; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Gene Expression Regulation; Protein Structure, Tertiary; Reproducibility of Results; Synaptic Transmission; Two-Hybrid System Techniques