OBJECTIVE: To evaluate whether participation in a four-month, pedometer-based, physical activity, workplace health program is associated with long-term sustained improvements in risk factors for type 2 diabetes and cardiovascular disease, eight-months after the completion of the program. DESIGN AND METHODS: A sample size of 720 was required. 762 Australian adults employed in primarily sedentary occupations and voluntarily enrolled in a workplace program were recruited. Demographic, behavioural, anthropometric and biomedical measurements were completed at baseline, four-months and twelve-months. RESULTS: 76% of participants returned at twelve-months. Sustained improvements at twelve-months were observed for self-reported vegetable intake, self-reported sitting time and independently measured blood pressure. Modest improvements from baseline in self-reported physical activity and independently measured waist circumference at twelve-months indicated that the significant improvements observed immediately after the health program could not be sustained. Approximately half of those not meeting guidelines for physical activity, waist circumference and blood pressure at baseline, were meeting guidelines at twelve-months. CONCLUSIONS: Participation in this four-month, pedometer-based, physical activity, workplace health program was associated with sustained improvements chronic disease risk factors at twelve-months. These results indicate that such programs can have a long-term benefit and thus a potential role to play in population prevention of chronic disease.