Sjövall, Fredrik1; Ehinger, Johannes K H2; Marelsson, Sigurður E2; Morota, Saori2; Asander Frostner, Eleonor2; Uchino, Hiroyuki2; Lundgren, Johan2; Arnbjörnsson, Einar2; Hansson, Magnus J2; Fellman, Vineta2; Elmér, Eskil2
1 Department of Intensive Care, Abdominal Centre, Rigshospitalet, The Capital Region of Denmark2 unknown
Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.