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cAMP biosensors applied in molecular pharmacological studies of G protein-coupled receptors

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Authors:
  • Mathiesen, Jesper Mosolff ;
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    Department of Drug Design and Pharmacology, Faculty of Pharmaceutical Sciences, Københavns Universitet
  • Vedel, Line ;
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    Medicinal Chemistry Research, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, Københavns Universitet
  • Bräuner-Osborne, Hans
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    Orcid logo0000-0001-9495-7388
    Medicinal Chemistry Research, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, Københavns Universitet
DOI:
10.1016/B978-0-12-407865-9.00011-X
Abstract:
Cyclic adenosine monophosphate (cAMP) is a common second messenger that mediates numerous biological responses. Intracellular cAMP levels are increased by activation of G(s)-coupled G protein-coupled receptors (GPCRs) and decreased by activation of G(i)-coupled GPCRs via the adenylyl cyclase. Many end-point assays for quantifying GPCR-mediated changes in intracellular cAMP levels exist. More recently, fluorescence resonance energy transfer (FRET)-based cAMP biosensors that can quantify intracellular cAMP levels in real time have been developed. These FRET-based cAMP biosensors have been used primarily in single cell FRET microscopy to monitor and visualize changes in cAMP upon GPCR activation. Here, a similar cAMP biosensor with a more efficient mCerulean/mCitrine FRET pair is described for use in the 384-well plate format. After cloning and expression in HEK293 cells, the biosensor is characterized in the 384-well plate format and used for measuring the signaling of the G(s)-coupled ß(2)-adrenergic receptor. The procedures described may be applied for other FRET-based biosensors in terms of characterization and conversion to the 384-well plate format.
Type:
Journal article
Language:
English
Published in:
Methods in Enzymology, 2013, Vol 522, p. 191-207
Main Research Area:
Medical science
Publication Status:
Published
Review type:
Undetermined
Submission year:
2013
Scientific Level:
Scientific
ID:
235795845

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