1 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU2 MEMPHYS Center, Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU3 Institute Max von Laue - Paul Langevin4 Université de Grenoble5 Université Libre de Bruxelles6 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU
The effect of terminally anchored chains on the structure of lipid bilayers adsorbed at the solid/water interface was characterized by neutron reflectivity. In the studied system, the inner leaflet, closer to the substrate, consisted of head-deuterated 1,2-distearoyl-sn-glycero-3-phosphorylcholine (DSPC) and the outer leaflet comprised a mixture of DSPC and polyethylene glycol (PEG) functionalized 1,2-distearoyl-sn-glycero-3-phosphoethanolamine. The DSPC headgroups were deuterated to enhance sensitivity and demarcate the bilayer/water interface. The effect on the inner and outer headgroup layers was characterized by w 1/2 , the width at half-height of the scattering length density profile. The inner headgroup layer was essentially unperturbed while w 1/2 of the outer layer increased significantly. This suggests that the anchored PEG chains give rise to headgroup protrusions rather than to blister-like membrane deformations.
European Physical Journal E. Soft Matter, 2013, Vol 36, Issue 1, p. 1-7