1 Department of Chemical and Biochemical Engineering, Technical University of Denmark2 The Danish Polymer Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark3 Department of Systems Biology, Technical University of Denmark4 Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark5 Department of Micro- and Nanotechnology, Technical University of Denmark6 Self-Organized Nanoporous Materials, Department of Micro- and Nanotechnology, Technical University of Denmark7 Infection Microbiology, Department of Biotechnology and Biomedicine, Technical University of Denmark8 Center for Nanostructured Graphene, Center, Technical University of Denmark
Biofilms cause extensive damage to industrial settings. Thus, it is important to improve the existing techniques and develop new strategies to prevent bacterial biofilm formation. In the present study, we have prepared nanoporous polymer films from a self-assembled 1,2-polybutadiene-b-polydimethylsiloxane (1,2-PB-b-PDMS) block copolymer via chemical cross-linking of the 1,2-PB block followed by quantitative removal of the PDMS block. Sodium dodecyl sulfate (SDS) was loaded into the nanoporous 1,2-PB from aqueous solution. The SDS-loaded nanoporous polymer films were shown to block bacterial attachment in short-term (3 h) and significantly reduce biofilm formation in long-term (1 week) by gram-negative bacterium Escherichia coli. Tuning the thickness or surface morphology of the nanoporous polymer films allowed to extent the anti-biofilm capability.
International Journal of Molecular Sciences (online), 2013, Vol 14, Issue 2, p. 3050-3064