1 Section of Poultry Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark2 Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark3 National Veterinary Institute, Technical University of Denmark4 Technical University of Denmark5 unknown
The epidemiology of an outbreak of Newcastle disease in a population of approximately 12 000 free-living pheasants (Phasianus colchicus) on the island of Faeno in Denmark in 1996 is described. The mortality epizootic demonstrated over an observation period of 3 weeks. A total of 70 avian paramyxovirus serotype 1 (APMV-1) isolates was made from the flock. The intra cerebral pathogenicity indices of the 4 isolates tested were in the range 1.78-1.88. By means of immunoperoxidase monolayer assay with murine monoclonal antibodies and sequence analysis of an RT-PCR amplified segment of the F-0 viral protein it was found, that thr virus belonged to the highly virulent C1 antigenic group and that the amino acid sequence at the F-0 cleavage site corresponded with the sequences of virulent APMV-1 strains. Based on the epidemiological circumstances it is believed that the virus mas transmitted to the pheasants by feral birds.
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 1999, Vol 46, Issue 6, p. 381-387