Vigsnæs, Louise Kristine1; van den Abbeele, Pieter7; Sulek, Karolina1; Frandsen, Henrik Lauritz1; Steenholdt, Casper5; Brynskov, Jørn5; Vermeiren, Joan7; van de Wiele, Tom7; Licht, Tine Rask6
1 National Food Institute, Technical University of Denmark2 Division of Food Microbiology, National Food Institute, Technical University of Denmark3 Division of Food Chemistry, National Food Institute, Technical University of Denmark4 Ghent University5 Copenhagen University Hospital6 Copenhagen Center for Health Technology, Center, Technical University of Denmark7 Ghent University
We compared fecal microbial communities derived either from Ulcerative Colitis (UC) patients in remission (n = 4) or in relapse (n = 4), or from healthy subjects (n = 4). These communities were used for inoculation of a dynamic in vitro gut model, which contained integrated mucin-covered microcosms. We found that the microbiota of the 'mucus' largely differed from that of the 'lumen'. This was partly due to decreased mucus-associated populations of lactic acid producing bacterial populations (LAB), as LAB originating from UC patients had a significantly decreased capacity to colonize the mucin-covered microcosms as compared to those originating from healthy subjects. We found significant differences between the metabolomes of UC patients in relapse and remission, respectively, while the metabolome of patients in remission resembled that of healthy subjects. These novel findings constitute an important contribution to the understanding of the complex etiology of UC.