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1 Neurologisk Afdeling N BFH, Bispebjerg and Frederiksberg Hospital, The Capital Region of Denmark 2 Clinical Intervention and Neuropsychiatric Schizophrenia Research/Center for Neuropsykiatrisk Skizofreniforskning, Psykiatrisk Center Glostrup, Mental Health Services, The Capital Region of Denmark 3 unknown 4 Psykiatrisk Center Glostrup, Mental Health Services, The Capital Region of Denmark 5 Institut for Farmakologi og Farmakoterapi
Decreased parvalbumin expression is a hallmark of the pathophysiology of schizophrenia and has been associated with abnormal cognitive processing and decreased network specificity. It is not known whether this decrease is due to reduced expression of the parvalbumin protein or degeneration of parvalbumin-positive interneurons (PV(+) interneurons). In this study we examined PV(+) expression in two rat models of cognitive dysfunction in schizophrenia, the environmental social isolation (SI) and pharmacological neonatal phencyclidine (neoPCP) models. Using a stereological method, the optical fractionator, we counted neurons, PV(+) interneurons, and glial cells in the medial prefrontal cortex (mPFC) and hippocampus (HPC). In addition, we quantified the mRNA level of parvalbumin in the mPFC. There was a statistically significant reduction in the number of PV(+) interneurons (p = 0.021) and glial cells (p = 0.024) in the mPFC of neonatal phencyclidine rats. We observed no alterations in the total number of neurons, hippocampal PV(+) interneurons, parvalbumin mRNA expression or volume of the mPFC or HPC in the two models. Thus, as the total number of neurons remains unchanged following phencyclidine (PCP) treatment, we suggest that the decreased number of counted PV(+) interneurons represents a reduced parvalbumin protein expression below immunohistochemical detection limit rather than a true cell loss. Furthermore, these results indicate that the effect of neonatal PCP treatment is not limited to neuronal populations. © 2012 International Society for Neurochemistry, J. Neurochem. (2012) 10.1111/jnc.12061.
Journal of Neurochemistry, 2013, Vol 124, Issue 1, p. 548-557
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