HROMOSOME translocation, which is a rearrangement of arms between two chromosomes, is a major group of chromosome abnormalities leading to cancer. As a result, two derivative chromosomes with sequences coming from both chromosomes are formed. The current translocation detection method is a Fluorescent In Situ Hybridization, which is laborious and involves use of expensive reagents . Here we present a label free technique for detection of chromosome translocations. As a proof of concept detection of chromosome translocation between chromosome 3 (Chr3) and chromosome 9 (Chr9) was chosen.