Skovstrup, Søren5; Le Quement, Sebastian Thordal2; Hansen, Thomas3; Jakobsen, Tim Holm4; Harmsen, Morten4; Tolker‐Nielsen, Tim6; Nielsen, Thomas Eiland7; Givskov, Michael Christian8; Taboureau, Olivier4
1 Organic Chemistry, Department of Chemistry, Technical University of Denmark2 Department of Chemistry, Technical University of Denmark3 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark4 Department of Systems Biology, Technical University of Denmark5 Technical University of Denmark6 University of Copenhagen7 Department of Organic Chemistry, Technical University of Denmark8 Department of Microbiology, Technical University of Denmark
With the widespread occurrence of bacterial resistance to antibiotics, the development of new strategies beyond conventional treatments is a pursuit taken by public health institutions worldwide. LasR, a transcription factor that controls quorum sensing in Pseudomonas aeruginosa, has emerged as an attractive therapeutic target for the next generation of antimicrobial agents. In the present study, a virtual screening workflow combining pharmacophore‐ and structure‐based approaches was used to identify new LasR ligands. Five novel inducers and three inhibitors of LasR activity were validated experimentally by use of a cell‐based assay. Interestingly, these compounds are molecularly distinct from the native signal molecule, N‐3‐oxododecanoyl‐L‐homoserine lactone (OHN), and may serve as lead structures for the design of new drugs. The binding modes of these compounds to the OHN binding site in LasR were predicted and used to identify the key interactions that contribute to the induction and inhibition of LasR activity.