1 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2 CFB - Core Flow, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3 CFB - Metagenomic Systems Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4 Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark5 Department of Systems Biology, Technical University of Denmark
The application of genome-scale technologies, both experimental and in silico, to industrial biotechnology has allowed improving the conversion of biomass-derived feedstocks to chemicals, materials and fuels through microbial fermentation. In particular, due to rapidly decreasing costs and its suitability for identifying the genetic determinants of a phenotypic trait of interest, whole genome sequencing is expected to be one of the major driving forces in industrial biotechnology in the coming years. We present some of the recent studies that have successfully applied high-throughput sequencing technologies for finding the underlying molecular mechanisms for (a) improved carbon source utilization, (b) increased product formation, and (c) stress tolerance. We also discuss the strengths and weaknesses of different strategies for mapping industrially relevant genotype-to-phenotype links including exploiting natural diversity in natural isolates or crosses between isolates, classical mutagenesis and evolutionary engineering.
Computational and Structural Biotechnology Journal, 2012, Vol 3, Issue 4