Pedersen, Lasse Eggers1; Fink, Dorte Rosenbek1; Jungersen, Gregers1
1 National Veterinary Institute, Technical University of Denmark2 Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark3 Adaptive Immunology & Parasitology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark
The genomic region (SLA) of the swine major histocompatibility complex (MHC), which bind and present endogenous peptides to circulating T cells of the immune system, is extremely polymorphic comprising high numbers of different alleles, many of which encode a distinct MHC class I molecule. Each SLA molecule is only able to bind a restricted number of peptides with specific biochemical characteristics matching important anchor positions in the peptide binding groove. Although the diversity of T cells is vast, the individual MHC make-up thus limits the range of potential T cell epitopes for any given individual. Therefore analyses of the prevalence of SLA alleles in a population are fundamental to employ pathogen-specific subunits or peptides in novel vaccines or immune diagnostics. In this study we present the use of low- and high-resolution PCR-based typing methods to identify individual and commonly expressed SLA class I alleles in Danish outbred swine. A total of 108 animals from eight different production herds were tested, and with low resolution sequence specific primer (SSP)-PCR typing the top five most commonly expressed SLA class I allele groups were found to be SLA-3*04XX, SLA-1*08XX, SLA-1*07XX, SLA-2*04XX, and SLA-1*04XX, respectively. Furthermore, customized high resolution primers were designed and used to identify specific alleles within the above mentioned allele groups and within the SLA-2*05XX group. In conclusion our studies suggest the most common haplotype in Danish pigs to be the Lr-4.0 expressing SLA-1*04XX, SLA-2*04XX and SLA-3*04XX.
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4th European Veterinary Immunology Workshop (EVIW), 2012