Rank, Christian1; Klejnstrup, Marie Louise1; Petersen, Lene Maj1; Kildgaard, Sara5; Frisvad, Jens Christian6; Gotfredsen, Charlotte Held4; Larsen, Thomas Ostenfeld7
1 Department of Systems Biology, Technical University of Denmark2 Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3 Organic Chemistry, Department of Chemistry, Technical University of Denmark4 Department of Chemistry, Technical University of Denmark5 unknown6 Fungal Chemodiversity, Department of Biotechnology and Biomedicine, Technical University of Denmark7 Natural Product Discovery, Department of Biotechnology and Biomedicine, Technical University of Denmark
Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.