B. Adhikari, Khem4; Lærke, Helle N.5; Mortensen, Anne G.4; Fomsgaard, Inge S.4
1 Department of Agroecology - Crop Health, Department of Agroecology, Science and Technology, Aarhus University2 Department of Animal Science - Molecular nutrition and reproduction, Department of Animal Science, Science and Technology, Aarhus University3 Department of Agroecology, Science and Technology, Aarhus University4 Department of Agroecology - Crop Health, Department of Agroecology, Science and Technology, Aarhus University5 Department of Animal Science - Molecular nutrition and reproduction, Department of Animal Science, Science and Technology, Aarhus University
Thorough knowledge of the absorption and metabolism of dietary benzoxazinoids is needed to understand their health-promoting eﬀects. In this study, the fates of these bioactive compounds were examined by LC-MS/MS in plasma, urine, and feces after ingesting a daily dose of 4780 ± 68 nmol benzoxazinoids from rye bread using Wistar rats as a model. HBOA-glc (2-β-D-glucopyranosyloxy-1,4-benzoxazin-3-one) was the predominant benzoxazinoid in the plasma (74 ± 27 nmol/L), followed by DIBOA-glc (2-β-D-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one) and HBOA. The total level of benzoxazinoids in the urine was 1176 ± 66 nmol/d, which corresponds to approximately 25% of the total dietary intake. The urinary benzoxazinoid proﬁle diﬀered from that of plasma with HBOA-glc and DIBOA-glc (647 ± 31 and 466 ± 33 nmol/d, respectively) as the major urinary components. The glucuronide conjugates of HBOA and DIBOA were detected in both the plasma and urine. Ndehydroxylation was found to be a critical step in the absorption of hydroxamic acids. This unprecedented study will trigger future interest in the biological eﬀects of benzoxazinoids in whole grain rye and wheat diets in humans and other animals.
Journal of Agricultural and Food Chemistry, 2012, Vol 60, Issue 46, p. 11518-11524