1 Department of Chemistry and Bioscience, The Faculty of Engineering and Science, Aalborg University, VBN2 Section of Biotechnology, The Faculty of Engineering and Science, Aalborg University, VBN3 The Faculty of Engineering and Science, Aalborg University, VBN4 Aarhus Universitet5 University of Tokyo6 University of Tokyo
The fusarielins are a group of metabolites found in several Aspergillus and Fusarium species that have been reported to have with weak antifungal, antibiotic and cytotoxic effects. This study identifies fusarielin A, F, G and H isolated from Fusarium as mycoestrogens. Mycoestrogens are compounds from fungi that bind to the estrogen receptors and induce an estrogenic response in targeted cells. All four tested fusarielins stimulate MCF-7 cell proliferation with fusarielin H as the most potent, able to stimulate cell proliferation 4-fold in a resazurin metabolism assay at 25 μM. MDA-MB-231 cells without the estrogen receptor-α and MCF-10a cells without estrogen receptors were not stimulated by fusarielins. Furthermore, the stimulation was prevented in MCF-7 cells when fusarielins were incubated in the presence of the estrogen receptor antagonist fulvestrant. These observations suggest that fusarielins bind to the estrogen receptor and act as weak mycoestrogens.
Toxicology Letters, 2012, Vol 214, Issue 3, p. 259-262
Breast cancer; Estrogen receptor; MCF-7; Mycoestrogen; Estrogen; Secondary metabolites; Fusarium; breast cancer; estrogen receptor; estrogen; secondary metabolites; fusarium