Holbech, Henrik3; Kinnberg, Karin Lund3; Petersen, Gitte2
1 Department of Biology, Faculty of Science, SDU2 DHI3 Department of Biology, Faculty of Science, SDU
This document presents the validation report (phase 2) of the Fish Sexual Development Test (FSDT). The Fish Sexual Development Test (FSDT) covers a life-stage where sexual development is particularly sensitive to perturbation caused by endocrine active chemicals. The chemical exposure lasts for about 60 days, at the end of which endpoints of ecological relevance like the sex ratio of the exposed fish is calculated and the biomarker endpoint vitellogenin is measured in individual animals. In 2003, Denmark, on behalf of the European Nordic countries, proposed a new project o develop a Test Guideline on the fish sexual development test to the Working Group of the National Coordinators of the Test Guidelines Programme (WNT). The project was included on the Test Guidelines workplan in 2003, and extensive validation of the test method was carried out until 2009. Two validation studies were performed, including chemicals representing various modes of action (oestrogen, (anti-)androgen, aromatase inhibitor) and negative chemicals. The chemicals tested in the phase 2 validation included the weakly active oestrogenic 4-tert-octylphenol and the androgenic dihydrotestosterone for the species medaka and zebrafish, and the weakly active oestrogenic 4-tert-pentylphenol, the anti-androgenic flutamide and the oestrogenic 17β-estradiol for the stickleback. The validation has been overseen by a validation management group for Eco-toxicity testing (VMG-eco) and a fish drafting group. A peer-review of the validation has been organised in 2011 and the report is available in the Series on Testing and Assessment as No.143. The draft validation report has been endorsed by the WNT at its meeting held on 12-14 April 2011. [The Joint Meeting of the Chemicals Committee and Working Party on Chemicals, Pesticides and Biotechnology agreed to its declassification on 22 June 2011].
Main Research Area:
Oecd Environment, Health and Safety Publications
Organisation for Economic cooporation and development, 2011