Münstermann, U3; Fritz, G3; Seitz, G3; Lu, Y P3; Schneider, H R3; Issinger, O G4
1 Faculty of Science, SDU2 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU3 unknown4 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU
Protein kinase CKII (i.e. casein kinase II, CKII, NII) is expressed at a higher level in rapidly proliferating tissues and in solid human tumours (e.g. colorectal carcinomas) when compared to the corresponding non-neoplastic colorectal mucosa. This could be shown by (a) Western blotting of cellular extracts from solid tumours followed by immunostaining with an anti-CKII polyclonal antibody, (b) immunohistochemical staining of cells from tissue sections and (c) by activity measurements using the CKII-specific synthetic peptide (RRRDDDSDDD). The maximum observed activity in the colorectal carcinomas investigated was up to eightfold higher in the tumour specimens than in the non-neoplastic tissue (i.e. colorectal mucosa). The activity range was between 33-350 U/mg protein and in the case of colorectal mucosa 13-106 U/mg protein. The amount of CKII determined in the individual tumours was in the range 0.4-1.6 nmol/g tissue.
European Journal of Biochemistry, 1990, Vol 189, Issue 2, p. 251-257
Breast; Breast Neoplasms; Carcinoma, Intraductal, Noninfiltrating; Casein Kinases; Colorectal Neoplasms; Female; Humans; Immunoenzyme Techniques; Immunohistochemistry; Intestinal Mucosa; Kinetics; Protein Kinases