Pultz, Dennis2; Bennetzen, Martin2; Rødkær, Steven Vestergaard2; Stefanko, Adam2; Andersen, Jens S.2; Ejsing, Christer S.2; Færgeman, Nils J.2
1 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU2 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU
Dietary restriction (DR) extends the life span of multiple species, ranging from single-celled organisms like yeast to mammals. This increase in longevity by dietary restriction is coupled to profound beneficial effects on age-related pathology. Despite the number of studies on DR and the physiological changes DR induces, only little is known about the genetics and signalling networks, which regulate the DR response. We have recently shown that inhibition of fatty acid synthesis in Saccharomyces cerevisiae induces autophagy mediated by TORC1 signalling and affects life span. In the present study, we have used quantitative mass spectrometry to further examine how inhibition of fatty acid synthesis affects cellular signalling events in Saccharomyces cerevisiae. We have identified approximately 2000 phosphorylation sites of which more than 400 have been identified as being regulated in a temporal manner in response to inhibition of fatty acid synthesis by cerulenin. By in silico analysis of these phosphorylation events, we have identified the major downstream regulated processes and signalling networks mediating the cellular response to fatty acid starvation. The analysis further identifies putative signalling components, which confer cerulenin-induced changes in the cellular lipidome.