Husen, Peter Rasmussen5; Fidorra, Matthias6; Hartel, Steffen4; Bagatolli, Luis7; Ipsen, John Hjort5
1 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU2 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU3 Fysisk Institut, Faculty of Science, SDU4 University of Santiago5 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU6 Fysisk Institut, Faculty of Science, SDU7 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU
Quantitative characterization of the lateral structure of curved membranes based on fluorescence microscopy requires knowledge of the fluorophore distribution on the surface. We present an image analysis approach for extraction of the fluorophore distribution on a spherical lipid vesicle from confocal imaging stacks. The technique involves projection of volumetric image data onto a triangulated surface mesh representation of the membrane, correction of photoselection effects and global motion of the vesicle during image acquisition and segmentation of the surface into domains using histograms. The analysis allows for investigation of the morphology and size distribution of domains on the surface.
European Biophysics Journal, 2012, Vol 41, Issue 2, p. 161-175