1 Det Sundhedsvidenskabelige Fakultet, SDU2 Patologisk Afdeling, Institute of Regional Health Research, Det Sundhedsvidenskabelige Fakultet, SDU3 unknown4 Institute of Pathology, Institute of Regional Health Research, Det Sundhedsvidenskabelige Fakultet, SDU5 Institute of Pathology, Institute of Regional Health Research, Det Sundhedsvidenskabelige Fakultet, SDU
OBJECTIVE: To proof the prognostic relevance of HPV L1 capsid protein detection on colposcopically-guided punch biopsies in combination with p16. STUDY DESIGN: Sections of colposcopically-guided punch biopsies from 191 consecutive cases with at least 5 years of follow-up were stained with HPV L1 capsid protein antibodies (Cytoactiv screening antibody) and a monoclonal anti-p16 antibody. Fifty sections were derived from a benign group, 91 from low-grade (cervical intraepithelial neoplasia [CIN 1]) lesions and 50 from high-grade (CIN 2 and 3) lesions. RESULTS: Overall only 16.1% of the 87 L1-negative, p16-positive CIN lesions showed remission of the lesion compared to 72.4% of the double positive cases. None of the L1/p16 double negative CIN lesions progressed. CONCLUSION: HPV L1 capsid protein detection with Cytoactiv screening antibody seems to be a promising new tool to predict the behavior of HPV-associated (p16-positive) early dysplastic lesions.
Analytical and Quantitative Cytology and Histology, 2008, Vol 30, Issue 2, p. 78-82