1 Det Sundhedsvidenskabelige Fakultet, SDU2 Paediatrics, Department of Clinical Research, Det Sundhedsvidenskabelige Fakultet, SDU3 Human Genetics, Department of Clinical Research, Det Sundhedsvidenskabelige Fakultet, SDU4 Human Genetics, Department of Clinical Research, Det Sundhedsvidenskabelige Fakultet, SDU
Inflammatory Bowel disease (IBD) is a condition characterised by chronic recidivous inflammation of the bowel and intestine. IBD includes chron´s disease (CD) and ulcerative colitis (UC). The combined prevalence of CD and UC are app. 1 in 500 in the general Caucasian population. In 25% of the cases IBD patients are diagnosed during childhood or adolescence. CD may involve any part of the gastrointestinal tract, where as UC usually are limited to rectal and colonic mucosal layers but may extend to the cecum. CD and UC are complex manifestations probably caused by a multitude of factors including environmental, microbial and genetics (3). As have been shown in twin studies and by the presence of strong familial aggregation. Especially CD but UC as well have strong genetic elements. The genetic component has been greatly emphasised recently through numerous Genome wide association studies (GWA), and microarray based expression studies. In IBD the increased production of chemo attractants from the inflamed microenvironment results in recruitment of activated CD4+ T lymphocytes which results in tissue damage. Where Th1 cell-derived cytokines has been reported to be essential mediators in CD with high (IFN)-IL-12 and IL-23 production the main affected leukocyte population in UC seems to be the Th2 –type with high production of IL-13, IL-5 and IL-4. These findings all point to a situation where similar clinical manifestations due to a diverse immunological response can lead to an inflammatory reaction based on the activation of different downstream pathways. Thus it seems that different genetic backgrounds can lead to similar clinical manifestations, and as well determines the susceptibility to IBD. In the previous micro array based expression studies on UC the main target has been to point to new candidate genes based on analysis of the main up or down regulated genes in the dataset. The majority of the studies are hampered by a relatively shortcoming of the numbers of genes analysed on the particular array. In this study the main target has been to point to clusters of genes involved in biochemical pathways with a changed regulation in order to understand the physiological, inflammatory and biochemical factors underlying the manifestation of UC.
Microarray; Ulcerative Colitis
Main Research Area:
BIT's 8th Annaul congress of International Drug Discovery Sciences and Technology, 2010