1 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU2 FLinT - Center for Fundamental Living Technology, Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU3 Center for Fundamental Living Technology (FLinT)4 Department of Biochemistry and Molecular Biology5 The Mærsk Mc-Kinney Møller Institute6 Department of Bioinformatics Engineering7 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU
Artificial vesicles have been used for decades as model systems of biological cells to investigate scientific questions in simulacra. In recent years, the significance of artificial vesicles further increased because they represent ideal candidates to become the building block of a de novo construction of a cell in a bottom-up manner. Numerous efforts to build an artificial cell that bridge the living and non-living world will most presumably represent one of the main goals of science in the 21st century. It was shown that artificial genetic programs and the required cellular machinery can be incorporated into vesicles, and therefore allow the synthesis of a large number of proteins (Noireaux et al. 2005). However, vesicle fission remains one of the upcoming challenges in the artificial cell project (Noireaux et al. 2011). So far, vesicle fission is implemented by applying mechanical stress to vesicles (Hanczyc et al. 2003). In the present work, we developed a scenario how a genetically controlled fission of vesicles may be achieved by the synthesis of a special class of viral proteins within artificial vesicles. Because the authors already have a lot of experience in the water-in-oil emulsion transfer method to prepare vesicles, the base for the development of a protocol to induce fission in artificial cell may be available.