Mohammed, Magdy M. D.7; Christensen, Lars Porskjær10; Ibrahim, Nabaweya A.7; Awad, Nagwa E.7; Zeid, Ibrahim F.8; Pedersen, Erik Bjerregaard11; Jensen, Kenneth Bendix11; Colla, Paolo L.9
1 Faculty of Science, SDU2 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU3 Institute of Chemical Engineering, Biotechnology and Environmental Technology, Faculty of Engineering, SDU4 Faculty of Engineering, SDU5 Dermato-venerology and Allergy Centre, Department of Clinical Research, Det Sundhedsvidenskabelige Fakultet, SDU6 NAC, Institut for Fysik og Kemi, Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU7 Pharmacognosy Department, National Research Center, Cairo8 Chemistry Department, Faculty of Science, El-Menoufia University, El-Menoufia9 Department of Science and Biomedical Technology, Cittadella University, Monserrato10 Institute of Chemical Engineering, Biotechnology and Environmental Technology, Faculty of Engineering, SDU11 Department of Physics, Chemistry and Pharmacy, Faculty of Science, SDU
In Proceedings of 4<sup>th</sup> Conference on Research and Development of Pharmaceutical Industries (Current Challenges)
As part of our screening of anti-AIDS agents from natural sources e.g. Ixora undulata, Paulownia tomentosa, Fortunella margarita, Aegle marmelos and Erythrina abyssinica, the different organic and aqueous extracts of Linum grandiflorum leaves and seeds were evaluated in vitro by the microculture tetrazolium (MTT) assay. The activity of the tested extracts against multiplication of HIV-1 wild type IIIB, N119, A17, and EFVR in acutely infected cells was based on inhibition of virus-induced cytopathicity in MT-4 cells. Results revealed that both the MeOH and the CHCl3 extracts of L. grandiflorum have significant inhibitory effects against HIV-1 induced infection with MT-4 cells. The MeOH extract of the leaves is more potent than other extracts against MT-4 cell cultures infected with the wild type HIV-1, strain IIIB with an ED50 of 46 ± 6 µM, while the CHCl3 extract of the seeds is more potent than other extracts against MT-4 cell cultures infected with the double mutation K103N+Y181C with an ED50 of 57 ± 4 µM.
Medicinal and Aromatic Plant Science and Biotechnology, 2009, Vol 3, Issue Special Issue 1, p. 37-41