1 Faculty of Science, SDU2 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU3 unknown4 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU
We have used electrospray mass spectrometry (ESMS) in combination with protein chemistry and genetics to delineate post-translational modifications in yeast of barley alpha-amylase 1 (AMY1), a 45 kD enzyme crucial for production of malt, an important starting material in the manufacture of beer and whisky. In addition to signal peptide processing these modifications are: (1) removal of C-terminal Arg-Ser by Kex1p, (2) glutathionylation of Cys95, (3) O-glycosylation, and (4) additional degradation of the C-terminus.
Nature Biotechnology, 1993, Vol 11, Issue 10, p. 1162-5
Base Sequence; Blotting, Western; Glutathione; Hordeum; Mass Spectrometry; Molecular Sequence Data; Mutation; Plasmids; Protein Folding; Protein Processing, Post-Translational; Recombinant Proteins; Saccharomyces cerevisiae; alpha-Amylase