1 The Department of Environmental, Social and Spatial Change, Roskilde University2 Environmental Dynamics, Department of Science and Environment, Roskilde University3 Department of Science and Environment, Roskilde University4 unknown
A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identify species.