1 Department of Pharmacy, Faculty of Pharmaceutical Sciences, Københavns Universitet2 Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, Københavns Universitet3 Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, Københavns Universitet4 Department of Pharmacy, Faculty of Pharmaceutical Sciences, Københavns Universitet
A genuine analytical method to determine native steroids (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17b-estradiol, and 17a-estradiol) and one anthropogenic steroid (ethynylestradiol) in environmental solid samples is presented. In summary, the sample preparation was systematically evaluated with testing different solvents and a suite of solid phase extraction materials. The optimized method utilizes the pressurized liquid extraction (PLE) technology with automated (integrated) clean-up combined with an additional off-line multi-step clean-up. The sample extracts were derivatized and analyzed utilizing a gas chromatography triple quadrupole mass spectrometry technology. This method is applicable on all types of environmental solid samples (e.g., biosolids, soils and sediments).