Gonzalez, Santiago F.2; Lukacs-Kornek, Veronika3; Kuligowski, Michael P.2; Pitcher, Lisa A.2; Degn, Søren Egedal8; Kim, Young-A2; Cloninger, Mary J.5; Martinez-Pomares, Luisa6; Gordon, Siamon7; Turley, Shannon J.3; Carroll, Michael C.2
1 Department of Medical Microbiology and Immunology, Faculty of Health Sciences, Aarhus University, Aarhus University2 Immune Disease Institute, Harvard Medical School3 Dana Farber Cancer Institute4 Department of Biomedicine - Forskning og uddannelse, Øst, Department of Biomedicine, Health, Aarhus University5 Department of Chemistry and Biochemistry, Montana State University6 School of Molecular Medical Sciences, University of Nottingham7 Sir William Dunn School of Pathology, University of Oxford8 Department of Biomedicine - Forskning og uddannelse, Øst, Department of Biomedicine, Health, Aarhus University
A major pathway for B cell acquisition of lymph-borne particulate antigens relies on antigen capture by subcapsular sinus macrophages of the lymph node. Here we tested whether this mechanism is also important for humoral immunity to inactivated influenza virus. By multiple approaches, including multiphoton intravital imaging, we found that antigen capture by sinus-lining macrophages was important for limiting the systemic spread of virus but not for the generation of influenza-specific humoral immunity. Instead, we found that dendritic cells residing in the lymph node medulla use the lectin receptor SIGN-R1 to capture lymph-borne influenza virus and promote humoral immunity. Thus, our results have important implications for the generation of durable humoral immunity to viral pathogens through vaccination.
Nature Immunology, 2010, Vol 11, Issue 5, p. 427-435