Denofrio, M. Paula2; Lorente, Carolina2; Breitenbach, Thomas4; Hatz, Sonja1; Cabrerizo, Franco M.2; Thomas, Andres H.2; Ogilby, Peter Remsen4
1 Department of Chemistry, Faculty of Science, Aarhus University, Aarhus University2 unknown3 Department of Chemistry, Science and Technology, Aarhus University4 Department of Chemistry, Science and Technology, Aarhus University
Pterins, heterocyclic compounds widespread in biological systems, participate in relevant biological processes and are able to act as photosensitizers. In the present study, we ascertained that 2-aminopteridin-4(3H)-one, abbreviated as Ptr, is readily incorporated into and ⁄ or onto cervical cancer cells (HeLa) and that these cells die upon UV-A irradiation of Ptr. Cell death was assessed using two tests: (1) the Rhodamine 123 fluorescence assay for mitochondrial viability and (2) the Trypan Blue assay for membrane integrity. The data suggest that, for Ptr-dependent photoinitiated cell death, events related to mitochondrial failure precede those associated with the failure of the cell membrane.
Photochemistry and Photobiology, 2011, Vol 87, p. 862-866