Jansakul, Chaweewan2; Tachanaparuksa, Kuldej3; Mulvany, Michael J.6; Sukpondma, Youwapa5
1 Department of Biomedicine - Pharmacology, Department of Biomedicine, Health, Aarhus University2 Department of Physiology, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand3 Department of Surgery, Hat-Yai Hospital, Hat-Yai 90110, Thailand4 Department of Biomedicine - Forskning og uddannelse, Øst, Department of Biomedicine, Health, Aarhus University5 Department of Chemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand6 Department of Biomedicine - Forskning og uddannelse, Øst, Department of Biomedicine, Health, Aarhus University
We have investigated effects and mechanisms responsible for the activity of 3, 5, 7, 30, 40-pentamethoxyflavone (PMF) on isolated human cavernosum. PMF is the major flavone isolated from Kaempferia parviflora claimed to act as an aphrodisiac. PMF caused relaxation of phenylephrine precontracted human cavernosal strips, and this effect was slightly inhibited by NG-nitro-L-arginine, a nitric oxide synthase inhibitor, but not by ODQ (soluble guanylate cyclase inhibitor), TEA (tetraethylammonium, blocker of voltage-dependent Kþ channels) or glybenclamide (blocker of ATP-dependent Kþ channels). PMF did not significantly inhibit the relaxant activity of glyceryltrinitrate or acetylcholine on human cavernosal strips precontracted with phenylephrine. In contrast, sildenafil (phosphodiesterase inhibitor) potentiated the relaxant activity of glyceryl trinitrate but not of acetylcholine. In normal Krebs solution with nifedipine (blocker of L-type Ca2þ channels), or in Ca2þ-free Krebs solution, PMF caused a further inhibition of human cavernosum contracted with phenylephrine. In human cavernosum treated with thapsigargin (inhibitor of sarcoplasmic reticulum Ca2þ-ATPase) in Ca2þ-free medium, PMF suppressed the concentration–response curve of human cavernosum to phenylephrine and a further suppression was found when SKF-96365 (a blocker of store-operated Ca2þ channels and Y-27632 (inhibitor of Rho-kinase)), but not nifedipine, were added sequentially. Thus, PMF had only a weak effect on the release of nitric oxide, and had no effect as a KATP- or KCa channel opener, a phosphodiesterase inhibitor, a store-operated Ca2þ channel blocker or a Rho-kinase inhibitor. Therefore, these studies suggest that PMF causes relaxation of human cavernosum through voltagedependent Ca2þ channels and other mechanisms associated with calcium mobilization.
European Journal of Pharmacology, 2012, Vol 691, p. 235-244