Shiguetomi Medina, Juan Manuel4; Kristiansen, Maja Sophie3; Ringgaard, Steffen5; Stødkilde-Jørgensen, Hans5; Møller-Madsen, Bjarne4
1 Department of Clinical Medicine - The Department of Orthopaedics E, ?AS, Department of Clinical Medicine, Health, Aarhus University2 Department of Clinical Medicine - The MR Research Centre, Department of Clinical Medicine, Health, Aarhus University3 The MR-Research Center. Aarhus University Hospital4 Department of Clinical Medicine - The Department of Orthopaedics E, ?AS, Department of Clinical Medicine, Health, Aarhus University5 Department of Clinical Medicine - The MR Research Centre, Department of Clinical Medicine, Health, Aarhus University
Purpose: Design and validation of an MR-based method that allows the calculation of the water content in cartilage tissue. Methods and Materials: Cartilage tissue T1 map based water content MR sequences were used on a 37 Celsius degree stable system. The T1 map intensity signal was analyzed on 6 cartilage samples from living animals (pig) and on 8 gelatin samples which water content was already known. For the data analysis a T1 intensity signal map software analyzer used. Finally, the method was validated after measuring and comparing 3 more cartilage samples in a living animal (pig). The obtained data was analyzed and the water content calculated. Then, the same samples were freeze-dried (this technique allows to take out all the water that a tissue contains) and we measured the water they contained. Results:The 37 Celsius degree system and the analysis can be reproduced in a similar way. MR T1 map based water content sequences can provide information that, after being analyzed using a T1-map analysis software, can be interpreted as the water contained inside a cartilage tissue. The amount of water estimated using this method was similar to the one obtained at the dry-freeze procedure. Conclusion: MR images obtained using T1 map based water content sequences provide information that can be analyzed to calculate the amount of water contained inside a cartilage tissue. This represents a non-invasive procedure that can provide information about inflammation or edema in cartilage and cartilage-like (e.g. Growth plate) tissue.