New genomic and transcriptomic methods greatly facilitate the study of the biology and evolution of fungal plant pathogens. The obligate biotrophic and asexually reproducing rust fungus Puccinia striiformis f.sp. tritici (Pst) forms haustoria during plant infection and delivers proteins and other effector molecules into the plant cell. Rust effectors take a key function in the rust-host interaction: on the one hand they are target of the plant resistance protein system and on the other hand they mediate virulence as subjects of diversifying selection. The virulence of Pst also depends on the presence and rapid evolution of such effectors. Pst causes yellow rust on wheat, one of the also depends on the presence and rapid evolution of such effectors. causes yellow rust on wheat, one of the most devastating diseases worldwide, and poses an imminent danger to previously resistant wheat varieties in unaffected geographic regions due to aerial long-distance dispersal and adaptation to a warmer climate. We are applying the next-generation Solexa/Illumina sequencing technology to both DNA and RNA of Pst. The assembled genomic and transcriptomic information is likely to facilitate the analysis of Puccinia striiformis effectors and to provide new insights into the molecular basis of virulence/avirulence as well as pathogen evolution.
12th International Cereal Rust and Powdery Mildews Conference Abstract Book, 2009
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12th International Cereal Rust and Powdery Mildews Conference, 2009