While the overall autonomic regulation of the heart and vasculature of anurans has been studied in some detail, little is known about the role of the adreno- and cholinoreceptors in smooth muscle when looking at resistance in the pulmonary and systemic vessels. Vascular smooth muscle is the primary site of regulation of resistance and therefore holds the capacity to control blood flow and pressure. This vascular control allows amphibians to regulate their shunt, i.e. partially bypassing either the pulmonary or systemic circuit. Shunting in amphibians has been studied due to their multiple respiratory modalities and their ontogeny including fundamental morphological changes during metamorphosis. Here we use wire myography to evaluate how the vascular tone of isolated blood vessels from the pulmocutaneous, pulmonary, cutaneous and systemic segments respond to sympathetic and parasympathetic stimulation. Hence, myography on vessels ranging from 0.2 to 2.0 mm allow for the investigation of isometric response to agonists and antagonists of smooth muscle with no autonomic tone. This provides the possibility of mapping the function of adreno- and cholinoreceptor in vascular regulation in anurans.
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Annual meeting of Society of Experimental Biology, SEB, 2011