Understanding cellular response to synthetic surfaces is of vital importance for such diverse areas as tissue engineering, medical implants, biosensors and cell therapy. Cells in vivo are surrounded by an Extra Cellular Matrix (ECM), which imposes spatial limitations and exhibits specific topographies and mechanical properties. Cells are also exposed to a number of biological moieties, such as soluble cytokines, growth factors and trans-membrane receptor ligands. Since all these factors affect the cells concomitantly, it is challenging to elucidate the effect of a single factor. Simplifying the complex 3 dimensional ECM into a 2 dimensional model system is a way to study the effect of topography or mechanical properties. Presented here is a method that combines nanometer and submicron scale features with micron scale features by combining photolithography and nanosphere lithography. This is accomplished by depositing a single layer of close packed polystyrene colloids onto a premade topographical structure. Subsequent modification has been performed using sputtering and evaporation of different physical species. The unmodified substrate is a topographical array with 169 different features, which previously has been screened with fibroblast and human dental pulp stem cells (hDPSC). Specific topography types have been identified for the inhibition of fibroblast growth . The tantalum covered surfaces presented here have been exposed to hDPSC.