1 Department of Biological Sciences, Microbiology, Faculty of Science, Aarhus University, Aarhus University2 Department of Bioscience - Microbiology, Department of Bioscience, Science and Technology, Aarhus University3 Department of Bioscience - Microbiology, Department of Bioscience, Science and Technology, Aarhus University
WHAT BACTERIA LEAVE BEHIND: BACTERIAL ORGANIC MATTER QUALITY AND BIOMARKER SIGNALS Knowledge of the cellular content of bacterial biomarkers (D-amino acids and muramic acid) provides us with a powerful tool to trace production and fate of bacterially derived organic matter. The biochemical composition of prokaryotic cells was studied by re-growing a mixed community of native sediment bacteria in anoxic sediment pore water. Cellular concentrations of L- and D-amino acids and amino sugars were determined by high performance liquid chromatography (HPLC). The proportion of Archaea, Gram positive and Gram negative Bacteria in the prokaryotic community were determined using catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH). The results showed that the L-amino acid composition of native sediment prokaryotes was similar to that of other organic matter sources. It is generally assumed that benthic prokaryotic communities are dominated by Gram negatives, although there is a lack of data supporting this assumption. This study provides insight into the biogeochemical makeup of mixed communities of sediment prokaryotes and evaluates the significance of Gram positives, Gram negatives and Archaea, in relation to prokaryotic contribution to sediment organic matter pools. Poster presentation Session #:053 Date: 01-29-09 Time: 18:00 - 20:00 Presentation is given by student: Yes
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ASLO Aquatic Sciences Meeting 2009: a cruise through Nice Waters.