Lipase-catalyzed acidolysis of canola oil with caprylic acid was performed to produce structured lipids. Six commercial lipases from different sources were screened for their ability to incorporate the caprylic acid into the canola oil. The positional distribution of FA on the glycerol backbone of unmodified oil and oil modified by Lipozyme RM IM, Lipozyme TL IM and Novozyme 435 were analyzed by ultra HPLC (UPLC). The results showed that Lipozyme RM IM from Rhizomucor miehei resulted in the highest caprylic acid incorporation and the lowest acyl migration so it was used in further studied. Reaction parameters studied included substrate mole ratio, enzyme load, reaction time and temperature. Incorporation of caprylic acid was higher when reactions were carried with 10% lipase of the total weight of substrates at a mole ratio of oil to caprylic acid of 1:4. The optimal time course and temperature for synthesis structured lipids were 15h and 50-60°C. Possible triacylglycerol species and physical properties of the SL product obtained at relative optimal conditions were characterized.