Fatty acids are specific compounds with well known chemical composition, but they exist in a great variety of molecules which differ in chemical and physical characteristics. Quantitative extraction of fatty acids is therefore a challenge. An acidic Bligh and Dyer method has been developed and compared with the traditional Stoldt fat extraction method. It is shown that depending on the matrix, the HCl-Bligh and Dyer extraction leads to 10-15% more total fatty acids and a 30-50% increase in polyunsaturated fatty acids compared with the official EU method. Besides being much more efficient for extraction of fatty acids, the HCl-Bligh and Dyer method is faster, and allows the inclusion of internal standard at the beginning of the sample treatment.
Lipid Technology, 2008, Vol 20, Issue 12, p. 280-281