A total of 154 phytoplasma strains from 15 ribosomal groups were employed for barcode sequences production. Besides strains in periwinkle, 36 strains in natural infected plants such as napier grass, grapevine, plum, jujube, apple, pear, spartium, pine tree, hibiscus and erigeron were employed. Barcode sequences were produced for 16SrDNA, tuf and SecA gene for 36 phytoplasma strains, while for 54 strains 16SrDNA sequences were obtained, and for 118 and 89 strains respectively the tuf and secA barcode. All obtained sequences and protocol for extraction and PCR amplification will be available in Qbank.
Bulletin of Insectology, 2011, Vol 64, Issue Supplement