Development of genomic resources in perennial grasses for high-throughput applications in genomic research and plant breeding has lagged behind most other crops. The predicted climate changes create novel patterns of biotic and abiotic stresses. Efficient characterization and utilization of genetic resources for developing improved cultivars adapted to the future climates depend on modern genomic resources. We will describe our recent activity in developing genomic resources for Festuca/Lolium and Phleum. These resources include the development and mapping of DArT-markers in Festuca pratensis, application of the DArTFest array in studying freezing tolerance in Festulolium, high-throughput EST sequencing (454-sequencing) targeting cold regulated genes in Festuca and Phleum, and the development of genic SNP markers for these species.