1 Obstetrics and Gynaecology, Faculty of Health Sciences, Aarhus University, Aarhus University2 Department of Human Genetics, Faculty of Health Sciences, Aarhus University, Aarhus University3 Department of Clinical Medicine - Department of Obstetrics and Gynaecology, Department of Clinical Medicine, Health, Aarhus University4 unknown5 Department of Clinical Medicine - Department of Obstetrics and Gynaecology, Department of Clinical Medicine, Health, Aarhus University
Physical exercise decreases the number of fetal cells in maternal blood J. M. Schlütter1, I. Kirkegaard1, B. Christensen2, S. Kølvraa3, N. Uldbjerg1 1. Department of Gynecology and Obstetrics, Aarhus University Hospital, Skejby, Aarhus N, Denmark. 2. FCMB ApS, Vejle, Denmark. 3. Department of Clinical Genetics, Vejle Hospital, Vejle, Denmark Objectives We have established a robust method to specifically identify and isolate a subgroup of fetal cells in maternal blood (fcmb) at a gestational age of 12 weeks. The concentration of these cells, however, varies considerably among pregnant women and 10-15 % have no cells in 30 mL, which is a challenge for the implementation of the method for prenatal diagnostic purposes. Considering the assumed fragility of circulating fetal cells we wondered if physical exercise prior to blood sampling might influence the number of fcmb. The objective of this study was therefore to assess whether physical activity within 24 hours prior to blood sampling affected the number of fcmb. Methods 59 women were included at a gestational age of 12 weeks. The number of fcmb was assessed in 30 mL blood processed by a proprietary method developed by FCMB ApS (limited liability company). Fetal cells in the blood, bound to fetal cell specific markers, were initially isolated by magnetic cell sorting, then stained with a cocktail of intracellular antibodies, identified and counted. Information about 6 variables reflecting the physical activity of the participants was obtained from questionnaires answered by the pregnant women just before blood sampling. Results Number of fcmb were significantly lower in women who had exercised for more than 30 minutes within the preceding 24 hours (median 1.5 vs. 3 fcmb; P = 0.04). The same tendency was found among 3 women who had used a bicycle for transport to the hospital as compared to transport by car (median 2 vs. 3 fcmb; P = 0.07). Even training of the pelvic floor within the preceding 3 hours seemed to slightly decrease fcmb (median 2 vs. 3 fcmb; P = 0.13). Conclusions Exercise within 24 hours reduces the number of fcmb in maternal blood. The explanation might be increased clearance of fcmb from the circulation during exercise. If analysis of fcmb is introduced as a clinical parameter pregnant women should avoid extensive physical activity 24 hours prior to blood sampling.
Foster celler; graviditet; Fysisk aktivitet; Fetal cells; Pregnancy; Exercise training
Main Research Area:
20th World Congress on Ultrasound in Obstetrics and Gynecology, 2010