1 Department of Anatomy, Faculty of Health Sciences, Aarhus University, Aarhus University2 Department of Clinical Medicine - The Department of Neurosurgery, Department of Clinical Medicine, Health, Aarhus University3 Department of Clinical Medicine - The Department of Neurosurgery, Department of Clinical Medicine, Health, Aarhus University
Despite the existence of many newer staining methods, Golgi staining still remains the primary method forvisualization of the dendrites and spines. The black deposit in the Golgi-Cox impregnated cells is a Mercuricsulphide, therefore autometallographic (AMG) technique which is used for visualization of the metals and metalsulphides/selenides in tissue may be used to enhance the Golgi-Cox staining. We demonstrated accordingly thatuse of AMG enhancement method on the Golgi-Cox staining gives good contrast and high resolution of dendritesand spines. Moreover, this method is cheaper and more flexible than conventional enhancement proceduresperformed with commercial photographic developers. The staining procedure is thoroughly described and wedemonstrate with qualitative and quantitative data, how Golgi-Cox immersion time and different AMGenhancement length may influence the staining of dendrites and spines in the rat hippocampus. The describedmethod will be of value for future behavioural-anatomical studies, examining changes in dendrite branching andspine density caused by brain diseases and their subsequent treatment.