Seeds of hexaploid wheat were treated with ethyl methanesulphonate (EMS). The M1 generation was grown in a greenhouse, and the individual shoots of the plants were classified and marked according to their ontogeny. The sterility in M1 and the frequencies of chlorophyll and viable mutants segregated in M2 were estimated. The frequency of chlorophyll mutants was very low and hence not very informative in regard to the mutation frequency in the individual spike categories. On the basis of the viable mutants segregated in M2, no significant difference in mutation frequency between the different spikes of hexaploid wheat after EMS treatment could be demonstrated.